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Novel Mammalian orthorubulavirus 5 Discovered as Accidental Cell Culture Contaminant



Mammalian orthorubulavirus 5 (PIV5), formerly named parainfluenza virus 5, resides within the Paramyxoviridae family [13]. The Paramyxoviridae family contains four subfamilies, Rubulavirinae, Avulavirinae, Metaparamyxovirinae, and Orthoparamyxovirinae. PIV5 is classified with seven other viruses in the Orthorubulavirus genus, one of two genera within Rubulavirinae. PIV5 is globally distributed and has been associated with respiratory disease in canine, cattle, swine, and lesser panda. While the pathogenesis of PIV5 in humans is unknown, both PIV5 and contagious Mumps orthorubulavirus belong to the Orthorubulavirus genus. Symptoms of Mumps orthorubulavirus in humans include fever, swollen salivary glands and possibly miscarriages or hearing loss.

We discovered a divergent Russian PIV5 strain (Moskva) in Vero cells exhibiting cytopathic eect (CPE). The cells were suspected to be accidentally contaminated by an ill laboratory technician, since the samples used for inoculation and the cells were negative for Moskva by PCR. In addition, Moskva contained a small hydrophobic (SH) protein coding sequence (CDS) present in human PIV5 strains.